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eppendorf mastercycler gradient instrument  (Eppendorf AG)


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    Structured Review

    Eppendorf AG eppendorf mastercycler gradient instrument
    Eppendorf Mastercycler Gradient Instrument, supplied by Eppendorf AG, used in various techniques. Bioz Stars score: 99/100, based on 200557 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mastercycle+instrument/Eppendorf/10__1016_slash_j__pedobi__2026__151140-133-6-10
    Average 99 stars, based on 200557 article reviews
    eppendorf mastercycler gradient instrument - by Bioz Stars, 2026-09
    99/100 stars

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    Related Articles

    Synthesized:

    Article Title: The Arabidopsis Abscisic Acid Catabolic Gene CYP707A2 Plays a Key Role in Nitrate Control of Seed Dormancy
    Article Snippet: .. First-strand cDNA was synthesized with random hexamers using a SuperScript first-strand synthesis system as described by Daniel-Vedele and Caboche (1993) using Moloney murine leukemia virus reverse transcriptase and random primers (Promega) or using the SuperScript first-strand synthesis system according to the manufacturer's instruction (Invitrogen). qRT-PCR was carried out using the first-strand cDNA as a template using the Mastercycle instrument (Mastercycle ep realplex; Eppendorf) with the RealMasterMix kit (Eppendorf) according to the manufacturer's protocol. .. Primers used for qRT-PCR for the CYP707A1 , CYP707A2 , and EF1α genes were those described previously ( Orsel et al., 2004 ; Seo et al., 2004 ; Millar et al., 2006 ).

    Article Title: A Spontaneous Deletion within the Desmoglein 3 Extracellular Domain of Mice Results in Hypomorphic Protein Expression, Immunodeficiency, and a Wasting Disease Phenotype
    Article Snippet: .. For real-time PCR quantification, cDNA synthesized using random primers from equal amounts of total RNA was analyzed using a Eppendorf Mastercycle Instrument (Eppendorf, Hamburg, Germany), SYBR Fast real-time quantitative PCR kits (KAPA Biosystems, Woburn, MA), and Dsg3-specific primers: 5′-CCAGACACACCAGCAACAATG-3′ (forward) and 5′-CAGCAGCACCACCATCAGG-3′ (reverse). ..

    Virus:

    Article Title: The Arabidopsis Abscisic Acid Catabolic Gene CYP707A2 Plays a Key Role in Nitrate Control of Seed Dormancy
    Article Snippet: .. First-strand cDNA was synthesized with random hexamers using a SuperScript first-strand synthesis system as described by Daniel-Vedele and Caboche (1993) using Moloney murine leukemia virus reverse transcriptase and random primers (Promega) or using the SuperScript first-strand synthesis system according to the manufacturer's instruction (Invitrogen). qRT-PCR was carried out using the first-strand cDNA as a template using the Mastercycle instrument (Mastercycle ep realplex; Eppendorf) with the RealMasterMix kit (Eppendorf) according to the manufacturer's protocol. .. Primers used for qRT-PCR for the CYP707A1 , CYP707A2 , and EF1α genes were those described previously ( Orsel et al., 2004 ; Seo et al., 2004 ; Millar et al., 2006 ).

    Reverse Transcription:

    Article Title: The Arabidopsis Abscisic Acid Catabolic Gene CYP707A2 Plays a Key Role in Nitrate Control of Seed Dormancy
    Article Snippet: .. First-strand cDNA was synthesized with random hexamers using a SuperScript first-strand synthesis system as described by Daniel-Vedele and Caboche (1993) using Moloney murine leukemia virus reverse transcriptase and random primers (Promega) or using the SuperScript first-strand synthesis system according to the manufacturer's instruction (Invitrogen). qRT-PCR was carried out using the first-strand cDNA as a template using the Mastercycle instrument (Mastercycle ep realplex; Eppendorf) with the RealMasterMix kit (Eppendorf) according to the manufacturer's protocol. .. Primers used for qRT-PCR for the CYP707A1 , CYP707A2 , and EF1α genes were those described previously ( Orsel et al., 2004 ; Seo et al., 2004 ; Millar et al., 2006 ).

    Quantitative RT-PCR:

    Article Title: The Arabidopsis Abscisic Acid Catabolic Gene CYP707A2 Plays a Key Role in Nitrate Control of Seed Dormancy
    Article Snippet: .. First-strand cDNA was synthesized with random hexamers using a SuperScript first-strand synthesis system as described by Daniel-Vedele and Caboche (1993) using Moloney murine leukemia virus reverse transcriptase and random primers (Promega) or using the SuperScript first-strand synthesis system according to the manufacturer's instruction (Invitrogen). qRT-PCR was carried out using the first-strand cDNA as a template using the Mastercycle instrument (Mastercycle ep realplex; Eppendorf) with the RealMasterMix kit (Eppendorf) according to the manufacturer's protocol. .. Primers used for qRT-PCR for the CYP707A1 , CYP707A2 , and EF1α genes were those described previously ( Orsel et al., 2004 ; Seo et al., 2004 ; Millar et al., 2006 ).

    Real-time Polymerase Chain Reaction:

    Article Title: A Spontaneous Deletion within the Desmoglein 3 Extracellular Domain of Mice Results in Hypomorphic Protein Expression, Immunodeficiency, and a Wasting Disease Phenotype
    Article Snippet: .. For real-time PCR quantification, cDNA synthesized using random primers from equal amounts of total RNA was analyzed using a Eppendorf Mastercycle Instrument (Eppendorf, Hamburg, Germany), SYBR Fast real-time quantitative PCR kits (KAPA Biosystems, Woburn, MA), and Dsg3-specific primers: 5′-CCAGACACACCAGCAACAATG-3′ (forward) and 5′-CAGCAGCACCACCATCAGG-3′ (reverse). ..



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